Fig. 4.

High CDCP1 expression in ccRCC is associated with poor survival. Tissue microarray provided by the Kidney Cancer Tissue Acquisition, Pathology and Clinical Data Core Facility at The Dana-Farber Cancer Institute. The anti-CDCP1 antibody (Cell Signaling Technology) was used 1:500 with TSA. Pictures were taken under 20× magnification on a Leica microscope. (A) Representative pictures of total CDCP1 levels in RCC tumors (low and high expression of CDCP1, left to right). (B) Histogram of IHC expression of CDCP1. Numbers on top of the columns indicate the number of patients. (C) Kaplan–Meier survival analysis for patients with low vs. high CDCP1 expression. DOD, date of diagnosis. (D) Lysates from RCC cell lines (RCC4, A498, and 786-0) were immunoblotted for the phosphorylation (P-734) of CDCP1, total CDCP1, HIF-2α, and α-tubulin for loading control under normoxia (21% O₂).