Fig. 1.

Abundance of MSRA4, MSRB1, and MSRB2 in seeds of M. truncatula. MSR abundance was measured by Western blot analysis in Jemalong seeds during maturation. For that purpose, soluble proteins (15 or 25 μg/lane), resolved by SDS/PAGE and transferred onto nitrocellulose membranes, were probed against anti-PtMSRA4, anti-AtMSRB1, or anti-AtMSRB2 sera (1/1,000). Quantification of bands detected on blots relative to bands stained with Coomassie blue in corresponding gels was performed using Quantity One (Bio-Rad) in two or three independent experiments. The abundance of bands in maturing seeds is expressed relative to that in mature seeds, stated as 100%.