Fig. 4.

Photoautotrophic growth of both hmox1 mutant and BVR-expressing transgenic lines of C. reinhardtii is severely impaired. (A) Comparative growth on agar medium of 4A+, hmox1, and two complemented hmox1 lines (ho1C1 and ho1C2) in the dark (with acetate, heterotrophic) or under light (∼180 µmol photons⋅m⁻²⋅s⁻¹, no acetate, photoautotrophic). (B) Comparative growth on agar medium of CC849 (wall-less wild type) and three BVR expression lines as in A. (C) Batch culture growth curves for hmox1 and complemented lines in continuous light (∼180 µmol photons⋅m⁻²⋅s⁻¹) inoculated at the same optical density (OD800 = 0.001, ∼1.0 × 10⁴ cells/mL). Bars indicate the SD of three to four replicates. (D) Suspension culture growth curves for the three BVR lines in continuous light as in C. (E) Normalized total chlorophyll (Chl a + Chl b) content per 10⁷ cells in 4A+, hmox1, and complemented ho1C1 and ho1C2 cell lines. Black bars, heterotrophic (with acetate) dark-grown samples; gray bars, photoautotrophic (no acetate) light-grown samples. (F) Normalized total chlorophyll in BVR lines as in E.