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. Author manuscript; available in PMC: 2013 Apr 24.
Published in final edited form as: FEBS Lett. 2012 Mar 24;586(8):1173–1178. doi: 10.1016/j.febslet.2012.03.036

Fig. 1.

Fig. 1

Two-hybrid analysis of interactions between Pta1 and Pap1. (A) Full-length Pta1 and Pta1 truncations used for two-hybrid analysis [16]. (B) The first 300 amino acids of Pta1 interact with the Pap1 N-terminus. Pta1 truncations were fused to GAD, while Pap1 and ΔNPap1 were fused to GBD. The GAD-Fip1 interaction confirms that ΔNPap1 is expressed. Tenfold serial dilutions of liquid cultures were spotted onto medium lacking leucine and tryptophan (CM-LT) to select for the two-hybrid plasmids. Activation of HIS3 expression (indicative of protein interaction) was tested by spotting the same strains onto medium lacking leucine, tryptophan and histidine (CM-LTH), followed by incubation for 2–3 days at 30 °C.