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. Author manuscript; available in PMC: 2013 Mar 4.
Published in final edited form as: J Mol Med (Berl). 2011 Apr 16;89(10):985–995. doi: 10.1007/s00109-011-0754-2

Fig. 5.

Fig. 5

Effect of aging on the expression of SDF-1 and HIF-1α in response to burn wounding. a SDF-1 mRNA expression in the burn wounds. RNA was isolated from the skin of non-burned mice (N) or from the burn wound (B) of young and old 129S1/SvImJ mice on day 2. SDF-1 RNA levels were analyzed by RT-qPCR. Mean±SEM (n=3–4 each) is shown. The overall effect of aging was assessed by two-way ANOVA (brackets at top); **P<0.01 vs Young N. b SDF-1 protein levels in serum samples collected from the same mice analyzed above were determined by ELISA. Mean±SEM (n=3–4 each) is shown. The overall effect of aging was assessed by two-way ANOVA (brackets at top); **P<0.01 vs Young N. c HIF-1α protein levels in burn wounds of young and old mice. On day 2 after burn, non-burned and burned tissues were harvested and aliquots of protein lysates were subjected to immunoblot assays using antibodies against HIF-1α and β-actin