Skip to main content
. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Toxicol In Vitro. 2012 Nov 23;27(2):662–671. doi: 10.1016/j.tiv.2012.11.013

Figure 5. Immunochemical identification of mono(GSH)-HDI as the major carbamoylating reaction product of GSH with vapor phase HDI.

Figure 5

HDI-specific Western blot was performed on albumin that had been co-incubated overnight at pH 9, with reverse phase HPLC purified GSH (lane 1), or equimolar amounts of mono(GSH)-HDI* (lane 2), or bis(GSH)-HDI (lanes 3), based on quantitation of HDA released by acid hydrolysis. Data shown are representative of N=3 independent experiments.