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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Toxicol In Vitro. 2012 Nov 23;27(2):662–671. doi: 10.1016/j.tiv.2012.11.013

Figure 6. Loci of HDI conjugation to human albumin, resulting from carbamoylation by GSH-HDI.

Figure 6

Sites of carbamoylation at pH 7 and/or pH 9, identified by MS/MS, are highlighted. K -conjugated w/ HDI* (*=2nd NCO hydrolyzed) resulting from pH 7 carbamoylation reaction overnight. Additional loci of conjugation resulting from carbamoylation reaction at pH 9 for 3 hr are also highlighted, including K (no underline)-conjugated w/ HDI* (*=2nd NCO hydrolyzed), K (double underline)-conjugated w/ HDI-GSH (GSH bound via γ-glu), or K̵ (underline and strikethrough)-peptide internally conjugated with HDI.