Figure 6. Loci of HDI conjugation to human albumin, resulting from carbamoylation by GSH-HDI.
Sites of carbamoylation at pH 7 and/or pH 9, identified by MS/MS, are highlighted. K -conjugated w/ HDI* (*=2nd NCO hydrolyzed) resulting from pH 7 carbamoylation reaction overnight. Additional loci of conjugation resulting from carbamoylation reaction at pH 9 for 3 hr are also highlighted, including K (no underline)-conjugated w/ HDI* (*=2nd NCO hydrolyzed), K (double underline)-conjugated w/ HDI-GSH (GSH bound via γ-glu), or K̵ (underline and strikethrough)-peptide internally conjugated with HDI.