Table 1. Primary antibodies.
| Target | Description | Specifity/Immunogen | Product nr and manufacturer | Use* |
|---|---|---|---|---|
| TP53 |
Rabbit polyclonal |
Against full-length human p53 fusion protein |
#9282 Cell Signalling Technology |
W, IF |
| OCT |
Mouse mAb |
Peptide raised against amino acids 1–134 of Oct-3/4 of human origin non-cross-reactive with Oct-3/4 isoforms B and B1. |
sc-5279, Santa Cruz |
W, IF, F |
| OCT4A/B |
Rabbit polyclonal, ChIP grade |
Peptide derived from within residues 300 to the C terminus of human Oct4. |
ab19857, Abcam |
W, IF, F |
| NANOG |
Mouse mAb, clone NNG-811 |
Against human Nanog. |
N3038, Sigma |
IF |
| P16INK4A |
Rabbit polyclonal |
Human p16, C-terminal. |
ab7962, Abcam |
IF |
| P16INK4A |
Rabbit polyclonal |
Raised against a peptide mapping at the N terminus of p16 of human origin. |
sc-467, Santa Cruz |
W, IF |
| Aurora B kinase |
Rabbit polyclonal |
Peptide derived from within residues 1–100 of human Aurora B. |
ab2254, Abcam |
IF |
| Telomerase reverse transcriptase (TERT) |
Mouse mAb |
Against human TERT. |
ab5181, Abcam |
IF |
| P62 (SQSTM1) |
Rabbit polyclonal |
Epitope corresponding to amino acids 151–440 of SQSTM1 of human origin. |
sc-25575, Santa Cruz |
IF |
| pCHK2 (phosphorylated T68) |
Rabbit polyclonal |
Epitope around the phosphorylation site of Threonine 68 (VST pQE) of human Chk2. |
ab38461, Abcam |
W, IF |
| α-tubulin |
Mouse mAb |
Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms. |
T5168, Sigma |
IF |
| γ-H2AX |
Rabbit polyclonal |
Recognizes mammalian, yeast, D. melanogaster and X. laevis γ-H2AX. |
4411-PC-100, Trevigen |
IF |
| RAD51 |
Mouse mAb |
Targeted to amino acids 1–138 of human Rad51. |
ab213, Abcam |
IF |
| LC3B |
Rabbit polyclonal |
Peptide derived from within residues 1–100 of human LC3B. |
ab63817, Abcam |
IF |
| P21CIP1 |
Rabbit polyclonal |
Raised against a peptide mapping at the C terminus of p21 of human origin. |
sc-397, Santa Cruz |
W, IF |
| GAPDH |
Mouse mAb, clone 6C5 |
Rabbit muscle GAPDH. |
ab8245, Abcam |
W |
| β-actin | Rabbit polyclonal | Synthetic peptide derived from within residues 1–100 of human β-actin. | ab8227, Abcam | W |
*
W, western; IF, immunofluorescent staining; f, flow cytometry.