Figure 3. Plasmid shuffle complementation tests of GPNs wild-type (WT) and mutants (E112K for yGPN1 and yGPN2 and E110K for yGPN3). Cells were grown on control (C) or 5-fluoroorotic acid (FOA) medium. Cells were incubated for 3 d at 30°C with different carbon sources, such as raffinose, raffinose/galactose (98/2 and 50/50) and galactose, to trigger an increasing induction of GPN proteins expression.