Figure 6. Effects of depleting survivin on apoptosis. HPNE-K-Ras, A549, Panc-1 and H460 cells were transfected with either control (NT) or survivin siRNA for 96 h, as described under “Materials and Methods.” Top panel: western blot analysis showing knockdown of survivin and cleaved PARP. Bottom panel: caspase-3 activity following depletion of survivin. Data are representative of two independent experiments.