Fig. 1.

The effect of inducing time on the expression amount of recombinant CagL and analysis of specificity of polyclonal antibody against CagL Expression of CagL was induced by 1 mmol/l IPTG. M protein mark, 1 negative control (PET28a(+) vector), 2–5E. coli BL21(DE3) containing PET28a(+)/cagL recombinant plasmid induced with IPTG after 2–5 h respectively, 6 Western blot analysis of CagL recombinant protein, 7 negative control, 8 Western blot analysis of serum of negative control, 9 Western blot analysis of polyclonal antibody against CagL