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. 2013 Mar 4;8(3):e57331. doi: 10.1371/journal.pone.0057331

Figure 3. The treatment of siFLIP abolished the synergistic effect of p22-FLIP on HBx-mediated NF-κB activation.

Figure 3

(A) A schematic representation of the siFLIP design. (B) The knock-down effect of siFLIP on p22-FLIP. The plasmid for p22-FLIP was co-transfected with siFLIP in Huh7 (left panel) and 293T cells (right panel). At 48 hours post-transfection, the expression level of p22-FLIP was analyzed by Western blot. (C–D) The effect of p22-FLIP knock-down on NF-κB activity. The indicated plasmids and pNF-κB-Luc (0.25 µg) were co-transfected with siFLIP (20 nM) or control siRNA in Huh7 cells(C) and 293T cells (D), respectively. Relative NF-κB activity was determined as described above. The expression levels of p22-FLIP and HBx were analyzed by western blot.