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. 2013 Mar 4;8(3):e57331. doi: 10.1371/journal.pone.0057331

Figure 4. Endogenous p22-FLIP is involved in HBx-mediated NF-κB signal.

Figure 4

(A) The effect of endogenous p22-FLIP on NF-κB activation. NF-κB activity was determined after co-transfection with pNF-κB-Luc and siFLIP (20 nM) with/without HBx-HA in Huh7 cells (left panel). The levels of mRNAs after treatment of siFLIP (right panel). HBx-HA was co-transfected with siFLIP with the indicated concentration. The expression levels were analyzed by semi-qRT-PCR using specific primers, respectively. GAPDH was used as a loading control. (B) The effect of c-FLIPL expression on HBx-mediated NF-κB activation. NF-κB activity was measured after co-transfection of pNF-κB-Luc and the indicated plasmids in Huh7 cells.