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. 2013 Mar 4;8(3):e58386. doi: 10.1371/journal.pone.0058386

Table 2. Effect of basal diet (B) and soybean oil (O) on the FAME composition (mg/g DM), FADME composition (µg/g DM) and partial sums (mg/g DM) of rumen content from lambs fed concentrate or lucerne, with or without soybean oil supplementation.

Diet1 P-values
C CO L LO B O B×O
FAME
12∶0 0.10bc ±0.02 0.15b ±0.02 0.07c ±0.01 0.24a ±0.03 0.096 <0.001 0.004
i-13∶0 0.01±0.00 0.03±0.01 0.03±0.00 0.04±0.01 0.030 0.028 0.176
13∶0 0.03ab ±0.00 0.03ab ±0.00 0.02b ±0.00 0.04a ±0.01 0.305 0.096 0.042
i-14∶0 0.08±0.01 0.09±0.04 0.14±0.01 0.15±0.02 0.018 0.574 0.886
14∶0 0.26b ±0.03 0.26b ±0.03 0.29b ±0.01 0.61a ±0.04 <0.001 <0.001 <0.001
i-15∶0 0.13±0.02 0.15±0.02 0.23±0.02 0.29±0.04 <0.001 0.146 0.455
a-15∶0 0.61a ±0.02 0.52ab ±0.08 0.28c ±0.02 0.41b ±0.04 <0.001 0.690 0.025
15∶0 0.27b ±0.04 0.19b ±0.03 0.38a ±0.02 0.51a ±0.08 <0.001 0.657 0.034
i-16∶0 0.10±0.02 0.11±0.05 0.12±0.01 0.16±0.02 0.239 0.266 0.589
16∶0 6.96b ±0.92 12.7a ±1.5 3.89c ±0.20 15.4a ±0.8 0.838 <0.001 0.007
i-17∶0 0.08ab ±0.01 0.06b ±0.01 0.08ab ±0.01 0.12a ±0.02 0.039 0.610 0.025
16∶1cis-7 0.13±0.04 0.08±0.02 0.08±0.01 0.10±0.01 0.397 0.542 0.133
16∶1trans-3 nd nd 0.05±0.00 0.06±0.01 − 0.160 −
16∶1cis-9 0.05b ±0.01 0.05ab ±0.01 0.04b ±0.00 0.07a ±0.01 0.322 0.031 0.033
a-17∶0 0.17a ±0.03 0.08bc ±0.01 0.07c ±0.01 0.11ab ±0.01 0.068 0.123 0.001
16∶0-3,7,11,15Me2 nd nd 0.02±0.01 0.19±0.03 − 0.001 −
17∶0 0.20b ±0.01 0.26ab ±0.03 0.15c ±0.01 0.31a ±0.01 0.723 <0.001 0.008
i-18∶0 0.04a ±0.00 0.03b ±0.00 0.02b ±0.00 0.05a ±0.01 0.352 0.070 <0.001
17∶1cis-9 0.03±0.00 0.04±0.02 0.01±0.00 0.02±0.00 0.212 0.273 0.949
Cyclo-173 0.17a ±0.02 0.09b ±0.01 0.04c ±0.00 0.15ab ±0.03 0.038 0.605 <0.001
18∶0 12.2±1.4 60.4±9.5 5.41±0.28 36.2±3.0 0.005 <0.001 0.095
18∶1trans 4 9.89c ±1.93 17.7b ±2.9 2.90d ±0.13 27.8a ±2.2 0.460 <0.001 <0.001
18∶1cis 5 3.48b ±0.51 4.81b ±0.66 1.17c ±0.07 9.75a ±0.98 0.050 <0.001 <0.001
18∶2n-6 1.88±0.23 3.48±0.70 1.07±0.05 4.45±0.72 0.879 <0.001 0.095
18∶2 non-conj.6 0.13d ±0.02 0.38c ±0.04 0.52b ±0.03 1.46a ±0.09 <0.001 <0.001 <0.001
18∶2 conj. (CLA)7 0.46b ±0.14 0.77b ±0.30 0.28b ±0.01 1.95a ±0.20 0.016 <0.001 0.001
18∶3n-3 0.11c ±0.02 0.28b ±0.06 0.82a ±0.05 0.71a ±0.09 <0.001 0.650 0.031
18∶0-oxo8 0.18±0.03 0.39±0.07 0.02±0.00 0.31±0.04 0.012 <0.001 0.350
19∶1 0.03b ±0.01 0.04b ±0.01 0.03b ±0.00 0.11a ±0.02 0.005 <0.001 0.004
20∶0 0.21±0.01 0.52±0.07 0.16±0.01 0.60±0.03 0.616 <0.001 0.093
20∶1cis-11 0.07b ±0.01 0.09ab ±0.01 0.02c ±0.00 0.11a ±0.01 0.190 <0.001 0.004
21∶0 0.03c ±0.00 0.04b ±0.00 0.03c ±0.00 0.06a ±0.01 0.069 <0.001 0.034
22∶0 0.15±0.01 0.44±0.05 0.17±0.01 0.57±0.02 0.011 <0.001 0.057
23∶0 0.05±0.01 0.08±0.01 0.09±0.00 0.14±0.01 <0.001 <0.001 0.097
24∶0 0.14c ±0.01 0.22b ±0.02 0.17b ±0.01 0.32a ±0.01 <0.001 <0.001 0.040
25∶0 0.03±0.00 0.04±0.01 0.03±0.00 0.05±0.01 0.687 0.014 0.317
26∶0 0.08b ±0.01 0.06bc ±0.01 0.06c ±0.00 0.11a ±0.01 0.034 0.044 <0.001
FADME
8∶0 0.87±0.11 1.42±0.25 nd Nd − 0.067 −
9∶0 4.53±1.81 17.0±6.0 1.50±0.47 7.23±2.54 0.069 0.012 0.327
10∶0 2.13±0.39 3.71±0.57 0.92±0.15 2.02±0.34 0.001 0.002 0.543
11∶0 4.12±1.17 4.95±1.17 1.12±0.24 2.04±0.44 0.008 0.400 0.961
12∶0 0.90±0.11 2.32±0.58 28.9±3.3 33.5±4.4 <0.001 0.281 0.563
16∶0 3.61±0.60 4.18±0.38 9.08±1.21 9.20±0.69 <0.001 0.665 0.781
Partial sums
∑FAME 38.4±2.2 105±13 19.0±0.7 103.8±4.1 0.156 <0.001 0.192
∑OBCFA 1.79b ±0.13 1.66b ±0.26 1.69b ±0.08 2.61a ±0.19 0.021 0.029 0.005
∑iso-FA 0.43±0.04 0.46±0.12 0.62±0.04 0.81±0.10 0.002 0.181 0.331
∑anteiso-FA 0.78a ±0.06 0.59ab ±0.09 0.35c ±0.02 0.52b ±0.05 <0.001 0.846 0.004
∑SFA 20.6±1.1 75.3±11.1 10.9±0.5 55.1±3.5 0.017 <0.001 0.379
∑C189 28.3±1.2 88.2±11.1 12.3±0.5 82.8±3.6 0.078 <0.001 0.374
∑BI10 11.2±2.02 21.3±3.1 4.20±0.16 34.4±2.4 0.175 <0.001 <0.001
∑FADME 0.02±0.00 0.03±0.01 0.04±0.00 0.05±0.01 <0.001 0.028 0.779

a,b,c,d within a row, means without a common letter differ (P<0.05);

1

Diets: C, concentrate; CO, concentrate plus 10% soybean oil; L, pelleted lucerne; LO, pelleted lucerne plus 10% soybean oil;

2

16∶0-3,7,11,15Me, 3,7,11,15-Tetramethylhexadecanoate;

3

cyclo-17, methyl-11-cyclohexylundecanoate;

4

18∶1trans, include 18∶1 trans-2 and trans-4 to trans-15;

5

18∶1cis; include 18∶1 cis-9 to cis-16;

6

18∶2non-conj., include trans-11,trans-15, cis-9,trans-13, cis-9,trans-12, trans-9,cis-12, trans-11,cis-15, cis-9,cis-15 and cis-12,cis-15;

7

18∶2conj. (CLA), conjugated isomers of linoleic acid presented on Table 3;

8

18∶0-oxo, include 10-O-18∶0, 13-O-18∶0 and 16-O-18∶0;

9

C18, includes all FA with 18 carbon chains;

10

BI, biohydrogenation intermediates, include total C18 FA minus 18∶0, 18∶1cis-9, 18∶1cis-11, 18∶2n-6 and 18∶3n-3. Abbreviations: i, iso; a, anteiso.