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. 2013 Mar 4;8(3):e58473. doi: 10.1371/journal.pone.0058473

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© 2013 Takase et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The HDAC immunolocalizations (green) were visualized with a neuronal marker (magenta) and Hoechst stain (blue). A: HDAC1 was detected in the nucleus (arrow) of a CRH neuron, with punctate immunoreactivity (arrowheads) in the PVN. B: HDAC1 was detected in the nuclei (arrows) of oxytocin neurons, with punctate immunoreactivity (arrowheads) in the PVN. C: HDAC1 was detected in the nuclei (arrows) of vasopressin neurons, with punctate immunoreactivity (arrowheads) in the PVN. D: HDAC1 was detected in the nucleus (arrow) of orexin neuron, with punctate immunoreactivity (arrowheads) in the LHA. E: HDAC1 was detected in the nucleus (arrow) of POMC neurons, with punctate immunoreactivity (arrowheads) in the ARC. F: HDAC1 was detected in the nuclei (arrows) of histamine neurons, with punctate immunoreactivity (arrowheads) in the TMN. G: HDAC1 was detected in the nuclei (arrows) of dopamine neurons. H: HDAC1 was detected in the nuclei (arrows) of serotonin neurons, with punctate immunoreactivity (arrowheads) in the DR. I: HDAC1 was detected in the nuclei (arrows) of noradrenaline neurons, with punctate immunoreactivity (arrowheads) in the LC. J: HDAC2 was detected in the nuclei (arrows) of CRH neurons. K: HDAC2 was detected in the nucleus (arrow) of oxytocin neuron. L: HDAC2 was detected in the nucleus (arrow) of vasopressin neuron. M: HDAC2 was detected in the nuclei (arrows) of histamine neurons. N: HDAC2 was detected in the nuclei (arrows) of dopamine neurons. O: HDAC2 was localized in the nuclei (arrows) of serotonin neurons in the DR. P: HDAC2 was detected in the nucleus (arrow) of noradrenaline neuron. Scale bars indicate 10 µm.