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. 2013 Mar 4;8(3):e57436. doi: 10.1371/journal.pone.0057436

Figure 2. Conditioned media of TWEAK-treated THP-1 macrophages inhibits HO-8910PM cell proliferation.

Figure 2

(A) TWEAK itself has no effects on HO-8910PM cell proliferation. Cultured HO-8910PM cells were either left untreated or treated with TWEAK (100 ng/ml) for 24, 48 and 72 hours, cell proliferation was measured by the CCK-8 assay. Absorbance value was expressed as percentage change of group marked as “&”. “n.s.”: non-statistical difference. (B) TWEAK and TNF-α co-administration inhibits HO-8910PM cell proliferation. Cultured HO-8910PM cells were either left untreated (“C”) or treated TNF-α (100 ng/ml) with or without indicated concentration of TWEAK for 24 and 72 hours, cell proliferation was measured by the CCK-8 assay. **P<0.05 vs. group “#”. (C) TWEAK promotes TNF-α secretion in cultured THP-1 macrophages. Cultured THP-1 cells were treated with indicated concentration of TWEAK for 24 h. TNF-α secretion was analyzed by ELISA. **p<0.05 vs. group “#”. (D) Conditioned media from TWEAK-treated THP-1 macrophages inhibits HO-8910PM cell proliferation. HO-8910PM cells were either cultured in RPMI or in conditional medium from TWEAK (different concentration)-treated THP-1 cells, cell proliferation was measured by the CCK-8 assay. Absorbance value was expressed as percentage change of group marked as “&”. *p<0.05.