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. 2013 Mar 4;8(3):e57318. doi: 10.1371/journal.pone.0057318

Figure 1. Internalization of ECP32–41 and EDN32–41.

Figure 1

(A) Beas-2B cells were incubated with 1, 5, 10, or 20 µM FITC-ECP32–41, FITC-EDN32–41, or FITC at 37°C for 1 h. The cells were washed twice with 500 µl PBS, trypsinized at 37°C for 15 min, suspended in 500 µl PBS, and then subjected to flow cytometry. (B) Beas-2B cells were incubated with 5 µM FITC-ECP32–41, FITC-EDN32–41, or FITC at 37°C for 5, 10, 30, or 60 min. The cells were then treated as described in (A) and subjected to flow cytometry. The results in (A) and (B) are expressed as the mean ± standard deviation (S.D.), n = 3. (C) Beas-2B cells were incubated with 5 µM FITC-ECP32–41, FITC-EDN32–41, or FITC at 37°C for 5 or 60 min before CLSM. Nuclei were stained with Hoechst 34850. Scale bar: 20 µm.