Figure 5. Cytotoxicity and membrane disruption by ECP^32–41.

Beas-2B cells were grown in serum-free medium in the presence of ECP^32–41 at indicated concentrations for 24 h. (A) The cytotoxic effect of ECP^32–41 was measured by MTT assay. The cell viability of untreated cells was set to 100%. Cells treated with 0.1% Triton X-100 was used as a positive control. (B) The membrane disruption by ECP^32–41 was measured by LDH assay. LDH released from cells lysed with 0.1% Triton X-100 in medium was defined as 100% leakage and LDH released from untreated cells was set as 0% leakage. The result is expressed as means ± S.D., n = 3. no significance (n.s.).