Figure 6. In vitro delivery of a protein and a peptide by ECP^32–41.

Beas-2B cells were incubated with 10 µM eGFP or eGFP-ECP^32–41 at 37°C for 1 h. The cells were then washed with 500 µl PBS, trypsinized at 37°C for 15 min, suspended in 500 µl PBS, and subjected to flow cytometry. (B) Beas-2B cells were treated with the indicated concentrations of TAT^47–57, ECP^32–41, KLA, KLA-TAT^47–57 or KLA-ECP^32–41 at 37°C for 24 h, and their cytotoxic effects were determined by the MTT assay. Cytotoxic effects of (C) KLA-TAT^47–57 and (D) KLA-ECP^32–41, at the indicated concentrations, on Beas-2B, A549, Caco-2, and AGS cells were assessed by the MTT assay after incubation at 37°C for 24 h. The cell viability untreated cells was set to 100%. The result is expressed as the mean ± S.D., n = 3.