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. 2013 Mar 4;8(3):e57428. doi: 10.1371/journal.pone.0057428

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© 2013 Tanaka et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Glutathione S-transferase (GST) pull-down assays revealed that Dullard formed a protein complex with Dvls in HEK293 cells. The association of Myc-tagged Dvls with the GST-Dullard recombinant protein (arrowheads) was detected by an anti-Myc antibody. CBB, Coomassie blue bands. (B) GST-Dullard with a D69E mutation in the catalytic domain, which abolishes its phosphatase activity, showed a binding activity with Myc-tagged Dvl2 similar to that of the wild-type (WT) Dullard protein. (C) Western blot analysis showing less Dvl2 protein in Dullard^−/− embryos. β-tubulin, loading control.