Figure 2. Let barcode 1 and 2 are the barcodes within the designed unique barcode set and another barcode containing errors introduced during the experiment, and the edges of the triangles represent the Levenshtein edit distance between them.

A) Barcode 1 can be converted to Barcode 3 with three operations. However two errors introduced either in barcode 1 or 2 can result in a new sequence, which requires same number of operations to transform to either barcode 1 or 2. Therefore, it cannot be classified. B) Barcode 1 is incorrectly synthesised or sequenced in such a way that it now has a smaller edit distance to barcode 2, which leads to its misclassification.