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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2012 Dec 12;8(1):287–300. doi: 10.1007/s11481-012-9426-4

Fig. 3.

Fig. 3

Cell viability in primary mouse astrocyte cell cultures stimulated for 4 hr with LPS 6.25 ng/ml and/or 12 mM sodium acetate, with 12 mM NaCl as control. Panel A shows the quantification of the ratio of secreted LDH in the media to total cellular LDH. All values represent the mean fold change relative to control ± SD (n = 6). Statistical significance was set at p 0.05 as determined by One Way ANOVA followed by Tukey’s post-hoc test. Panel B shows confocal microscopic images of primary astrocyte cultures used for TUNEL assay (n = 5), with fluorescin-dUTP-labeled fragmented DNA of apoptotic cells (green), the nuclei counterstained by propidium iodide (red), and co-localization of the fragmented DNA and nuclei (yellow). Positive control group received treatment with DNase I 50 Units/ml (n = 4).