Figure 1.

The conformational shifts between Na_vRh and Na_vAb. (A) Lateral rotation of the VSDs between Na_vRh and Na_vAb when the pore domains are superimposed. A cytoplasmic view is shown. Please refer to Supplementary information, Movie S1 for a morph of the overall conformational changes between Na_vRh and Na_vAb. (B) Structural changes between individual protomers when the structures of the tetrameric Na_vRh and Na_vAb are superimposed relative to the pore domains. Na_vRh and Na_vAb are colored blue and grey, respectively. (C) Dissection of the conformational shifts of the VSD segments relative to the pore domain. The S6 segments are shown in each panel as the reference to indicate the orientation of the structures. (D) The conformational shifts of the S4-5 helix as well as the S5 segment relative to the pore. A cytoplasmic view and a side view are shown. (E) The S4-5 helices and S5 segments are shifted (indicated by the black arrows) between Na_vRh and Na_vAb. The pore domain of Na_vRh is colored the same as in (C), and that of Na_vAb is colored silver. The orange arrows indicate the potential pushing force exerted from the adjacent protomer during the conformational changes of its VSD.