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. 2012 Dec 25;23(3):394–408. doi: 10.1038/cr.2012.178

Figure 3.

Figure 3

Functional impairment of CCL5-deficient MDSCs in vitro. (A, B) Splenocytes from CL4 transgenic mice stimulated in vitro with 1 μg/ml of HA512-520 peptide were cultured with various amounts of purified MO-MDSCs (A) or PMN-MDSCs (B) from CCL5 WT or KO mice. Percentages of proliferating cells were determined by flow cytometry (n = 3). (C) ELISPOT analysis for IFNγ production by activated T cells in the same experimental setup as in A. The ratio of MDSCs to CL4 splenocytes was 1:4. (D) Tumor-specific IFNγ production by T cells. Splenocytes from 4T1 tumor-bearing WT and KO mice were incubated with irradiated 4T1 cells for 48 h. IFNγ production by T cells was assessed by ELISPOT. Data are represented as mean ± SE. Each group contained five mice. *P < 0.05; **P < 0.01.