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. 2013 Feb 12;23(3):329–339. doi: 10.1038/cr.2013.22

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Copyright © 2013 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0

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Pericentric 5hmC is maintained through female germ cell development. (A) Representative images of E16.5 pachytene stage oocytes co-stained with 5hmC (red) and a synaptonemal complex marker SYCP3 (green). Germ cells are classified into three types based on the number of large 5hmC foci. Type I, less than 4 large foci; type II, five to eight foci; type III, more than nine foci. (B) Percentages of each group in E16.5 and E18.5 PGCs. Note that the distribution of the three groups is not significantly altered between E16.5 and E18.5 PGCs. (C) Representative images of 5hmC (red) and H4K20me3 (green), a pericentric heterochromatin marker, in germinal vesicle breakdown (GVBD), and metaphase II (MII) oocytes.