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. 2012 Nov 14;33(3):356–364. doi: 10.1038/jcbfm.2012.166

Figure 6.

Figure 6

Reactive microglia express and translocate sulfonylurea receptor 1 (SUR1) to the cell surface. (A) Confocal photomicrographs of SUR1 and Kir6.2 (green) in reactive microglia (CD11b-positive; red) localized to the medial striatum in middle cerebral artery occlusion rats. Yellow in the merge image denotes colocalization, whereby reactive CD11b-postive cells expressed SUR1 or Kir6.2 72 hours after ischemia. (B) Localization of glibenclamide (Gbc) (Gbc BODIPY FL; green fluorescence) in rat microglial primary culture. Non-activated or cultures activated with lipopolysaccharide (LPS)+interferon gamma (IFNγ) for 48 hours are shown in the upper row. Microglial cells were labeled with an anti-CD11b (red) antibody and Hoechst (blue) to stain the nuclei. Lower row shows respective colocalization of the red and green channels, where the yellow denotes the presence of the Gbc binding in microglial cells. Arrowhead denotes perinuclear colocalization and arrows show surface labeling. The data shown are representative of four experiments each. Scale bar in (A) is 15 μm and in (B) is 20 μm.