Figure 3.

Angiopoietin-2 (Ang2) receptors are expressed in primary cultures of neurons but Ang2 does not protect neurons against excitotoxic death. (A) Immunodetection of Tie2 receptor and β1 integrin on neuronal cultures. Scale bar: 20 μm. (B) Lack of effect of Ang2 on excitotoxic cell death induced by a treatment with N-methyl-𝒟-aspartic acid (NMDA) in neuronal cell cultures (12 DIV) assessed by the concentration of lactate dehydrogenase (LDH) released. Mean±s.d., n=3. *P<0.05 versus NMDA, Fisher protected protected least significant difference (PLSD) following a significant analysis of variance. (C) Representative T2-weighted magnetic resonance imaging (MRI) images showing that Ang2 does not protect against excitotoxic death in vivo.