Figure 5.

Hyperbaric oxygen (HBO) preconditioning upregulates SirT1 protein after oxygen–glucose deprivation (OGD) injury in primary cortical neurons. Representative western blot bands and quantitative evaluation of SirT1 protein expression (n=3/group). The expression of SirT1 was detected by immunoblots 24 hours after re-oxygenation. The expression of SirT1 was normalized to the expression of β-actin. Data were presented as mean±s.d. of three repeated experiments, one-way analysis of variance (ANOVA) followed by the least significant difference (LSD) test. *P<0.01 versus control; ^#P<0.01 versus HBO preconditioning. OGD: neurons subjected to oxygen-glucose deprivation for 2 hours; HBO: neurons subjected to HBO preconditioning for 2 hours (3.5 atmospheres absolute, >98% O₂); HBO +OGD: neurons subjected to OGD 24 hours after the end of HBO preconditioning.