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. 2012 Dec 19;33(3):428–439. doi: 10.1038/jcbfm.2012.187

Figure 2.

Figure 2

Rarefaction of CD31-immunoreactive endothelium after middle cerebral artery occlusion (MCAO). (A) Series of representative confocal microscopic images showing loss of CD31-immunoreactive endothelium after ischemia. (B) Quantification of vessel lengths based on CD31 immunofluorescence in the ischemic versus nonischemic hemisphere. A significant reduction of vessel length is observed at days 5 and 7 after MCAO. (C) Cleaved caspase-3 (CC3) immunohistochemistry and nuclear morphology reveal apoptosis of CD31+ endothelial cells at day 5 after ischemia (arrowhead). Upper left inset shows nuclear fragmentation of the CD31+ endothelial cell (arrow), lower right inset shows the orthogonal reconstruction obtained from the plane indicated (dashed line), showing CC3 immunoreactivity within the CD31+ endothelial cell. Bars represent means±s.d. *P<0.05 versus control mice. n=5, 4, 4, 5, 3, 5, and 5 for control, 1, 3, 5, 7, 14, and 28 days, respectively. Scale bars: 20 μm.