Skip to main content
. 2013 Mar 4;200(5):667–679. doi: 10.1083/jcb.201212095

Figure 5.

Figure 5.

Notochord vacuoles require vacuolar H+-ATPase for formation and maintenance. (A–F) Live confocal images of 4-dpf WT (A, C, and E) and mutant (B, D, and F) larvae dyed with MED. (B) H+-ATPase mutant atp6v1e1bhi577aTg. (D) H+-ATPase mutant atp6v1fhi1988Tg. (F) H+-ATPase mutant atp6v0cahi1207Tg. Arrows indicate fragmented vacuoles. (G–H’) Live confocal images of embryos treated with either DMSO or 500 nM bafilomycin for 4 h before vacuole inflation until the 20 ss, visualized with MED (G and H) or membrane GFP (G’ and H’). Live confocal images of embryos treated with either DMSO or 500 nM bafilomycin for 4 h, after vacuole formation had begun, until the 28 ss, visualized with MED (I and J) or membrane GFP (I’ and J’). v labels vacuoles inflating in DMSO control and red arrowheads label nuclei. Bars, 50 µm.