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. 2013 Mar 4;200(5):635–650. doi: 10.1083/jcb.201208150

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© 2013 Hayase et al.

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Figure 1. — Morg1 interacts with Par6. (A) The yeast two-hybrid interaction between Morg1 and Par6. PJ69-4A cells transformed with a pair of pACT2–Morg1 and pGBK encoding Par6α, Par6β, or Par6γ were grown in the absence of histidine and adenine. (B and D) Proteins in the lysate of COS-7 cells expressing indicated proteins (Cell lysate) were immunoprecipitated (IP) and then analyzed by immunoblot (Blot) with the indicated antibodies. (C) Proteins in lysates of MDCK cells were immunoprecipitated with the anti-Morg1 or control IgG, and then analyzed by immunoblot with the indicated antibodies. The arrowhead indicates endogenous Par6β; asterisk denotes the heavy chain of IgG.