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Summary of the purification process.
| Fraction sample | Soluble protein |
|---|---|
| Wet weight of cells | 5.379 g |
| Soluble total protein | 150 mg |
| Purified His-FIP-fve | 29.1 mg |
| Purity a (%) | 97.1 |
The purity of His-FIP-fve was assessed by densitometry analysis of Tris-Tricine SDS-PAGE. Total protein mass was estimated using a protein assay kit (Pierce) with BSA as a standard. One liter of culture media was used to purify His-Fip-fve.
