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. 2013 Jan 24;14(2):2258–2281. doi: 10.3390/ijms14022258

Figure 9.

Figure 9

EPOR resensitization following 3 IU/mL EPOα-, 3 IU/mL DarbEPO- or 5 IU/mL CERA-induced receptor desensitization. HUVECs were pre-treated with medium alone (control) or 3 IU/mL EPOα (A), 3 IU/mL DarbEPO (B) or 5 IU/mL CERA (C) for 18 or 54 min to induce receptor desensitization. The cells were then washed and replaced in medium alone for different periods of time (2 to 24 h) to allow for receptor resensitization. All samples were then stimulated by the addition of 3 IU/mL EPOα, 3 IU/mL DarbEPO or 5 IU/mL CERA for 5 min, and STAT-5 phosphorylation levels were quantified. The data are expressed as the percent STAT-5 phosphorylation compared to the untreated control cells (set to 100%) and represent the mean ± SEM of three different experiments. ** p < 0.01; * p < 0.05 vs. DES’.