Figure 5.

Transmission electron micrographs of the different leaf ages with Agrobacterium invasion in intercellular spaces. Leaf explants were infected with Agrobacterium at OD₆₀₀ ranging from 0.8 to 1.0 and infected for 20 min to 30 min. The leaf explants were cut transversely. (a) and (b) Uninfected leaf as the control; (c) and (d) 30-day-old leaf explant; (e) and (f) 60-day-old leaf explant; (g) and (h) 90-day-old leaf explant. In c–h, Agrobacterium invasion into the intercellular space was indicated by white circles and arrows. The magnified images of the boxes in the left column (a, c, e, g) were placed in the right column (b, d, f, h), respectively. Scale bar 20 μm.