Time course of raw sugarcane trash hydrolysis. (□) T. reesei crude extract, enzyme load per gram of substrate 14.5 FPU, 185 U CMCase, 15 U Avicelase and 0.1 U cellobiase; (△) C. graminicola crude extract, enzyme load per gram 3.6 FPU, 12.5 U CMCase, 2.0 U Avicelase, 342 U cellobiase, 225 U β-xylosidase, 1557 U xylanase and 5.0 U α-arabinofuranosidase; (●) mixture of T. reesei and C. graminicola crude extracts, enzyme load per gram 14.6 FPU, 155 U CMCase, 13.6 U Avicelase, 342 U cellobiase, 225 U β-xylosidase, 1557 U xylanase and 5.0 U α-arabinofuranosidase. Enzymatic assays were performed on duplicate aliquots, and the values shown represent averages from triplicate experiments (n = 3).