Table 3.
Experimental conditions and results of the statistical experimental design for β-xylosidase production by C. graminicola.
| Run | Real(Coded) values | |||
|---|---|---|---|---|
|
| ||||
| w | y | z | β-xylosidase a (U g−1) | |
| 1 | 1(−1) | 7(−1) | 1.5(−1) | 93.8 |
| 2 | 5(1) | 7(−1) | 1.5(−1) | 85.6 |
| 3 | 1(−1) | 7(−1) | 1.5(−1) | 99.6 |
| 4 | 5(1) | 7(−1) | 1.5(−1) | 104.2 |
| 5 | 1(−1) | 9(1) | 2.5(1) | 76.8 |
| 6 | 5(1) | 9(1) | 1.5(−1) | 72.6 |
| 7 | 1(−1) | 9(1) | 2.5(1) | 60.6 |
| 8 | 5(1) | 9(1) | 2.5(1) | 85.4 |
| 9 | 3(0) | 6(−1.68) | 2.0(0) | 56.0 |
| 10 | 3(0) | 11(+1.68) | 2.0(0) | 58.6 |
| 11 | 3(0) | 8(0) | 1.0(−1.68) | 59.6 |
| 12 | 3(0) | 8(0) | 3.0(+1.68) | 86.0 |
| 13 | 0.5(−1.68) | 8(0) | 2.0(0) | 40.2 |
| 14 | 7(+1.68) | 8(0) | 2.0(0) | 117.4 |
| 15 | 3(0) | 8(0) | 2.0(0) | 126.0 |
| 16 | 3(0) | 8(0) | 2.0(0) | 123.0 |
| 17 | 3(0) | 8(0) | 2.0(0) | 124.6 |
Notes: w: peanut hulls (%, w/w); y: time (days); z: initial moisture (mL g−1). Initial moisture of the culture media expressed as wet basis moisture content corresponded to: 46.9% (1 mL g−1), 55.8% (1.5 mL g−1), 63.5% (2.0 mL g−1), 68.6% (2.5 mL g−1) and 73.0% (3.0 mL g−1).
a
Response values for each run were the means of triplicate experiments, and each enzymatic assay was carried out in duplicate.