Table 4.

Experimental conditions and results of the statistical experimental design for xylanase production by C. graminicola.

Run	Real(Coded) Values
	x	y	z	k	s	Xylanase (U g−1)
1	25(−1)	6(−1)	1.5(−1)	0.5(−1)	0.7(1)	110.6
2	25(−1)	6(−1)	1.5(−1)	1.5(1)	0.3(−1)	70.9
3	25(−1)	6(−1)	2.5(1)	0.5(−1)	0.3(−1)	166.3
4	25(−1)	6(−1)	2.5(1)	1.5(1)	0.7(1)	140.4
5	25(−1)	10(1)	1.5(−1)	0.5(−1)	0.3(−1)	161.0
6	25(−1)	10(1)	1.5(−1)	1.5(1)	0.7(1)	128.9
7	25(−1)	10(1)	2.5(1)	0.5(−1)	0.7(1)	146.8
8	35(1)	10(1)	2.5(1)	1.5(1)	0.3(−1)	190.5
9	35(1)	6(−1)	1.5(−1)	0.5(−1)	0.3(−1)	39.1
10	35(1)	6(−1)	1.5(−1)	1.5(1)	0.7(1)	43.2
11	35(1)	6(−1)	2.5(1)	0.5(−1)	0.7(1)	146.8
12	35(1)	6(−1)	2.5(1)	1.5(1)	0.3(−1)	156.9
13	35(1)	10(1)	1.5(−1)	0.5(−1)	0.7(1)	16.4
14	35(1)	10(1)	2.5(−1)	0.5(−1)	0.3(−1)	22.8
15	35(1)	10(1)	2.5(1)	0.5(−1)	0.3(−1)	197.9
16	35(1)	10(1)	2.5(1)	1.5(1)	0.7(1)	122.3
17	20(−2)	8(0)	2.0(0)	0(1.0)	0.5(0)	32.3
18	40(2)	8(0)	2.0(0)	1.0(0)	0.5(0)	0.6
19	30(0)	4(−2)	2.0(0)	1.0(0)	0.5(0)	25.2
20	30(0)	12(2)	2.0(0)	1.0(0)	0.5(0)	337.1
21	30(0)	8(0)	1.0(−2)	1.0(0)	0.5(0)	75.4
22	30(0)	8(0)	3.0(2)	1.0(0)	0.5(0)	290.2
23	30(0)	8(0)	2.0(0)	0.0(−2)	0.5(0)	370.5
24	30(0)	8(0)	2.0(0)	2.0(2)	0.5(0)	373.5
25	30(0)	8(0)	2.0(0)	1.0(0)	0.1(−2)	375.8
26	30(0)	8(0)	2.0(0)	1.0(0)	0.9(2)	299.0
27	30(0)	8(0)	2.0(0)	1.0(0)	0.5(0)	352.8
28	30(0)	8(0)	2.0(0)	1.0(0)	0.5(0)	337.3
29	30(0)	8(0)	2.0(0)	1.0(0)	0.5(0)	364.7
30	30(0)	8(0)	2.0(0)	1.0(0)	0.5(0)	355.7
31	30(0)	8(0)	2.0(0)	1.0(0)	0.5(0)	349.9
32	30(0)	8(0)	2.0(0)	1.0(0)	0.5(0)	361.1

Notes: x: temperature (°C); y: time (days); z: initial moisture (mL g⁻¹); k: milled corncob (%, w/w); s: ammonium sulfate concentration (%, w/w). Initial moisture of the culture media expressed as wet basis moisture content corresponded to: 46.9% (1 mL g⁻¹), 55.8% (1.5 mL g⁻¹), 63.5% (2.0 mL g⁻¹), 68.6% (2.5 mL g⁻¹) and 73.0% (3.0 mL g⁻¹).

^aResponse values for each run were the means of triplicate experiments, and each enzymatic assay was carried out in duplicate.