Table 6.
Experimental conditions and results of the statistical experimental designs for β-glucosidase, β-xylosidase and xylanase activities.
| Run | β-glucosidase a | β-xylosidase a | Xylanase a | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| pH | T (°C) | U mg−1 | pH | T (°C) | U mg−1 | pH | T (°C) | U mg−1 | |
| 1 | 4.5(−1) | 55(−1) | 35.7 | 4.5(−1) | 55(−1) | 31.5 | 4.5(−1) | 60(−1) | 40.3 |
| 2 | 6.0(+1.41) | 65(0) | 27.6 | 6.0(+1.41) | 65(0) | 21.7 | 4.5(−1) | 70(1) | 46.2 |
| 3 | 5.5(1) | 55(−1) | 35.6 | 5.5(1) | 55(−1) | 27.7 | 6.5(1) | 60(−1) | 37.6 |
| 4 | 4.0(−1) | 75(1) | 36.3 | 4.5(−1) | 75(1) | 24.6 | 6.5(1) | 70(1) | 41.6 |
| 5 | 5.5(1) | 75(1) | 30.9 | 5.5(1) | 75(1) | 23.5 | 4.0(−1.41) | 65(0) | 43.4 |
| 6 | 5.0(0) | 65(0) | 51.1 | 5.0(0) | 65(0) | 32.3 | 7.0(+1.41) | 65(0) | 37.0 |
| 7 | 5.0(0) | 50(−1.41) | 27.8 | 5.0(0) | 50(−1.41) | 24.0 | 5.5(0) | 55(−1.41) | 48.3 |
| 8 | 5.0(0) | 65(0) | 51.2 | 5.0(0) | 65(0) | 31.1 | 5.5(0) | 75(+1.41) | 48.4 |
| 9 | 4.0(−1.41) | 65(0) | 32.5 | 4.0(−1.41) | 65(0) | 28.0 | 5.5(0) | 65(0) | 48.2 |
| 10 | 5.0(0) | 80(+1.41) | 24.9 | 5.0(0) | 80(+1.41) | 20.0 | 5.5(0) | 65(0) | 48.4 |
| 11 | 5.0(0) | 65(0) | 50.2 | 5.0(0) | 65(0) | 31.9 | 5.5(0) | 65(0) | 48.7 |
Note:
a
Response values for each run were the means of four experiments, and each enzymatic assay was carried out in triplicate.