Table 2.
cDNA/EST library parameters on the basis of sequencing of 500 recombinant clones from “Biqi” fruit at mature green (MG), pink ripe (PR), red ripe (RR), and full ripe (FR) stages.
| Library parameter | MG | PR | RR | FR |
|---|---|---|---|---|
| Titer of original cDNA library | 2.01 × 105 | 1.49 × 105 | 3.92 × 105 | 1.18 × 106 |
| Titer of EST library | 3.13 × 109 | 3.04 × 109 | 3.53 × 109 | 3.95 × 109 |
| Recombinant rate | 94.74% | 100% | 94.74% | 94.74% |
| Average length of EST (bp) | 701 | 597 | 565 | 1272 |
| Number of TCs | 14 | 37 | 28 | 53 |
| Number of singletons | 45 | 202 | 36 | 79 |