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. 2013 Feb 6;14(2):3376–3394. doi: 10.3390/ijms14023376

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© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Expression and clustering analysis of differentially expressed genes encoding epigenetic chromatin modification enzymes in pediatric ALL and normal control samples. Clustering analysis of the gene expression data from the real-time PCR array. The comparative C_t method was used for quantification of gene expression. The gene expression levels for each target gene were normalized to the housekeeping gene GAPDH within the same sample (−ΔC_t); then the relative expression of each gene (n = 87) was calculated using 10⁶ × Log₂(−ΔC_t). Gene expression in the normal karyotype B cell pediatric acute lymphoblastic leukemia (ALL) (n = 18) and control samples (n = 20) was analyzed using Multi Experiment View (MEV) clustering software.