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Illustration of the dewetting test for DPPC. The sample bilayer in (A), which is very similar to the bilayer in Figure 9C, was dewetted instantaneously and immediately rehydrated (B); The dewetting step was repeated a second time (C). The holes in the bilayer become progressively larger after each successive wash. The test proves that the sample in (A) was a complete bilayer with partially fused lipid patches on top.
