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A representative force–distance curve for the rupture of DOPC in water. The rupture occurs at FB = 3.0 nN. The tip first senses the bilayer at a distance zA–D = 6.8 nm from the mica surface. The rupture depth occurs at zB–D = 4.9 nm. The bilayer depth for DOPC is in good agreement with values reported by X-ray diffraction [54].
