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. 2013 Feb 6;14(2):3514–3539. doi: 10.3390/ijms14023514

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© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Tip induced DOPC gathering effects on sparsely covered samples indicating both the fluidity of DOPC and the weak attraction of DOPC with mica. (A) DOPC sample prepared with a very sparse coverage of DOPC; (B) and (C) successive scans of sample in A cause the lipid to be drawn together, making larger patches; (D) The effect is most clearly seen when a corner at the boundary between a previously scanned region, and an untouched region is scanned; (E) The distribution of pixel heights shows two distinct populations, representing the mica surface and the surface of the bilayer. The bilayer depth is found by subtracting the Gaussian fitted peaks of the two populations. The value of 5 nm obtained here agrees well with expected values.