Table 1.
HPLC-MS/MS mobile phase gradient elution condition.
| Time (min) | A (H2O) | D (Acetonitrile) | Flow (mL/min) |
|---|---|---|---|
| 0 | 85% | 15% | 0.4 |
| 0.5 | 85% | 15% | 0.4 |
| 6.0 | 10% | 90% | 0.4 |
| 7.0 | 10% | 90% | 0.4 |
| 7.1 | 85% | 15% | 0.4 |
| 9 | 85% | 15% | 0.4 |
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