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. 2013 Feb 8;14(2):3802–3816. doi: 10.3390/ijms14023802

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© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

(a) Determination of stable virally transduced SKOV3-TR30-m-PTIP-Sponge all cell line and its vector-only control SKOV3-TR30-m-PTIP-GFP by Fluorescence Microscope. (100×) 1, 2, 3: transduced by m-PTIP-GFP empty plasmid 1, white field, 2, with fluorescence, 3, the integration of 1 and 2. 4,5,6: transduced by m-PTIP-Sponge all inhibit plasmid, 4, white field, 5, with fluorescence, 6, the integration of 4 and 5; (b) Determination of stable virally transduced SKOV3-TR30-m-PTIP-Sponge all cell line and its vector-only control SKOV3-TR30-m-PTIP-GFP using Flow Cytometry. Ratio of cells expression green fluorescent protein for SKOV3-TR30, SKOV3-TR30-m-PTIP-GFP and SKOV3-TR30-m-PTIP-Sponge all were 0.00, 97.48% and 99.79%; (c) Plasmid Restriction Enzyme Digestion Results 1: m-PTIP-GFP empty plasmid, 2: m-PTIP-Sponge all inhibit plasmid, M: Marker.