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. 2013 Feb 8;14(2):3802–3816. doi: 10.3390/ijms14023802

Figure 7.

Figure 7

(a) The samples from SKOV3 cells and SKOV3-TR30 cells were subjected to Western Blot analysis with a BIM and PTEN antibody while β-actin levels were used as loading control; (b, c) The samples from SKOV3-TR30-m-PTIP-Sponge all cells and its vector-only control SKOV3-TR30-m-PTIP-GFP cells were subjected to Western Blot with BIM and PTEN antibody while GAPDH levels were used as loading control; (b) Decreased expression of miR-17~92 upregulates the expression of BIM instead of PTEN in SKOV3-TR30 cells without treatment of paclitaxel. 1,3 Protein samples from SKOV3-TR30 cells transduced by m-PTIP-GFP empty plasmid. 2,4 Protein samples from SKOV3-TR30 cells transduced by m-PTIP-Sponge all inhibit plasmid; (c) Decreased expression of miR-17~92 upregulates the expression of BIM instead of PTEN in SKOV3-TR30 cells after the treatment of paclitaxel with different concentration.1, 3, 5, Protein samples from SKOV3-TR30 cells transduced by m-PTIP-Sponge all inhibit plasmid 2,4,6: Protein samples from SKOV3-TR30 cells transduced by m-PTIP-GFP empty plasmid. The concentration of paclitaxel: 1,2: 20 nM, 3,4: 100 nM, 5,6: 200 nM.