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High-performance liquid chromatography (HPLC) profile obtained by re-analyzing peak 5. The protein was eluted using a linear gradient of acetonitrile in 0.1% trifluoroacetic acid (TFA). Absorbance was monitored at 214 nm. (A) Peptide-G2 (PG-2) peaks; (B) Tricine gel electrophoresis of purified PG-2: lane 1, molecular size markers (same as in Figure 1); lane 2, purified PG-2 (10 μg).
