Figure 4.

Representative images of Oil Red O (ORO) staining to visualize lipid droplets on liver sections (6 μm thick) from chow fed rats (a) and HFD control rats (b) treated with saline (5 mL/kg of body weight), and HFD rats treated with rosiglitazone (RSG, 3 mg/kg of body weight, (c)) or SGE (2 g/kg of body weight, (d)). Images from the stained liver were taken at a magnification of 200x. The scale bar represents 50 μM. Lipid droplets quantified at least 5 different high-power fields, being blind by two independent assessors. The intensity of staining of liver tissue with ORO provides a qualitative measure of the lipid contents (e). Data are means ± SEM, n = 6–8, ^† P < 0.05, ^‡ P < 0.01 versus chow control; *P < 0.05 and **P < 0.01 compared with HFD-Con.