Figure 7. Recruitment and Survival of Ag-Specific CD8⁺T Cells during VSV-OVA Infection.

(A) Serum concentrations of CCL4 in VSV-OVA-infected mice 24 hr p.i. Data are from three experiments.

(B) Purified CD8⁺ T cells from OT-I TCR Tg mice were CFSE labeled and adoptively transferred into BDCA2-DTR Tg mice. Mice were depleted and infected as described in Figure 6. Splenocytes isolated at6or22 hr p.i. were stained with anti-Vα2then analyzed by flow cytometry. Dot plots show the frequencies of CFSE⁺ cells among Vα2⁺ cells. Data are representative of three to eight mice per group.

(C) Mice were injected with 2 × 10⁶ CFSE-labeled CD8⁺ T cells from OT-I mice then infected in the footpad with VSV or VSV-OVA (1 × 10⁶ pfu) 24 hr later. Controlateral (CLN) and draining (DLN) lymph nodes were stained with anti-Vα2 and analyzed by flow cytometry at 9 or 25 hr p.i. Data are representative of three mice per group.

(D) Mice were injected with CFSE- or eFluor 670-labeled OT-I cells, then depleted or not and infected as described in Figure 6. Splenocytes were stained with anti-CD8, anti-Vα2, and Annexin V or CaspACE FITC-VAD-FMK 45 or 66 hr p.i. Bar graphs show the frequencies of OT-I cells among CD8⁺Va2⁺ cells (top) and frequencies of apoptotic OT-I cells (bottom). Data are from two experiments (mean ± SEM, n = 4–8).

p value, unpaired, two-tailed Student's t test.