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. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: Cell. 2013 Feb 28;152(5):1021–1036. doi: 10.1016/j.cell.2013.01.052

Figure 6. TAF3 Binding is Required for RNAPII Recruitment and Correlates with H3K4me3 Accumulation at the p21 and BTG2, but not the DR5 and FAS, Promoters.

Figure 6

(A) HCT116 (p53+/+ and p53−/−) cells were treated with doxorubicin (for 0, 2, 4, 8, 12, and 24 h) and qRT-PCR was performed to measure the rapid p53-dependent induction of the cell cycle genes p21 and BTG2 and the delayed induction of the proapoptotic genes DR5 and FAS. The expression levels after doxorubicin treatment are provided relative to the levels before doxorubicin. Error bars denote the standard deviation from duplicate reactions by real time PCR. (B, D) ChIP analyses with the indicated antibodies were performed using HCT116 cells that were treated with doxorubicin for 0, 4, 8, and 24 h. The amplicons used for real-time PCR are shown in the schematic of the p53 loci. ChIP for H3K4me3 was normalized to H3. An average of two independent ChIP experiments that are representative of at least three is shown with error bars denoting the standard deviation. (C) HCT116 cells were treated with non-targeting (control) or TAF3 siRNA. Following 8 h of doxorubicin, cell lysates were analyzed by ChIP with the indicated antibodies. An average of two independent ChIP experiments that are representative of at least three is shown with error bars denoting the standard deviation. See also Figure S5.